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Gastroenterology. 1997 Jan;112(1):163-73.

Phenotypes of B and T cells in human intestinal and mesenteric lymph.

Author information

1
Laboratory for Immunohistochemistry and Immunopathology, University of Oslo, National Hospital, Norway.

Abstract

BACKGROUND & AIMS:

Cells in lymph draining the human gut have not been characterized previously. The aim of this study was to phenotype B and T cells present in microlymphatics of Peyer's pathces and in mesenteric lymph.

METHODS:

The studies were conducted by multicolor immunohistochemistry, flow cytometry, and immunocytochemistry.

RESULTS:

In decreasing order of frequency, microlymphatics in Peyer's patches contained naive T (CD3+CD45RA+ alpha 4 beta 7low) and B (sIgD+CD20+ alpha 4 beta 7low) lymphocytes, memory T (CD45RO+ alpha 4 beta 7+) and B (sIgD-CD20+ alpha 4 beta 7+) lymphocytes, and B-cell blasts (CD19+CD38high alpha 4 beta 7high). Naive cells were usually positive for L-selectin, memory cells were either positive or negative, and B-cell blasts were usually negative. Mesenteric lymph contained naive T (approximately 60%) and B (approximately 25%) lymphocytes, memory T and B lymphocytes (approximately 10%), and B-cell blasts (approximately 2%). Cytospins confirmed these results and showed, in addition, that B-cell blasts contained cytoplasmic immunoglobulin (Ig) A, IgM, or IgG in overall proportions of 5:1: < 0.5.

CONCLUSIONS:

Our results are similar to the phenotypes previously described in animal thoracic or mesenteric lymph. A fraction of the B cells stimulated in Peyer's patches are near terminal differentiation (contain cytoplasmic Ig) before they enter peripheral blood. Many memory cells, and most if not all B-cell blasts entering lymph show an adhesion molecule profile (alpha 4 beta 7high L-selectin low) in keeping with the presumed phenotype of lymphoid cells destined for mucosal effector sites such as the gut lamina propria.

PMID:
8978355
[Indexed for MEDLINE]

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