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FEBS Lett. 1996 Dec 2;398(2-3):259-64.

Cloning and expression of full length mouse utrophin: the differential association of utrophin and dystrophin with AChR clusters.

Author information

1
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63110, USA. aguo@pharmdec.wustl.edu

Abstract

We have cloned and sequenced mouse utrophin cDNA, and successfully expressed full length utrophin (400 kDa) in both muscle and non-muscle cells. The expression of recombinant utrophin is compared with that of its homologue, dystrophin (427 kDa). We demonstrate that recombinant utrophin is targeted into agrin-induced acetylcholine receptor (AChR) clusters, while recombinant dystrophin is evenly distributed along cell membranes in cultured Sol 8 muscle cells. This observation suggests that utrophin and dystrophin may interact with different cytoskeletal proteins. The C-terminal domains are found to be responsible for the association of utrophin with AChR clusters.

PMID:
8977119
DOI:
10.1016/s0014-5793(96)01216-1
[Indexed for MEDLINE]
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