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FEBS Lett. 1996 Dec 2;398(2-3):159-64.

Presence of nitric oxide synthase activity in roots and nodules of Lupinus albus.

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Departamento de Fisiología y Bioquímica Vegetal, Centro de Ciencias Medioambientales, CSIC, Madrid, Spain.


NO is a widespread messenger molecule in physiology. We were interested in investigating whether an NO-generating system could be present in plants. NO and L-[14C]citrulline were synthesized by roots and nodules of Lupinus albus in an L-arginine-dependent manner. L-[14C]Citrulline production was inhibited by N(G)-monomethyl-L-arginine, a nitric oxide synthase antagonist, in a competitive way. NADPH-diaphorase activity was localized in the vascular bundles in root and nodules, and also in the nodule infected zone. This staining was significantly reduced in the presence of N(G)-monomethyl-L-arginine. These results indicate the presence of a putative nitric oxide synthase in plants.

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