Format

Send to

Choose Destination
Biophys J. 1996 Dec;71(6):3157-66.

A voltage-dependent role for K+ in recovery from C-type inactivation.

Author information

1
Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085, USA.

Abstract

Recovery from C-type inactivation of Kv1.3 can be accelerated by the binding of extracellular potassium to the channel in a voltage-dependent fashion. Whole-cell patch-clamp recordings of human T lymphocytes show that Ko+ can bind to open or inactivated channels. Recovery is biphasic with time constants that depend on the holding potential. Recovery is also dependent on the voltage of the depolarizing pulse that induces the inactivation, consistent with a modulatory binding site for K+ located at an effective membrane electrical field distance of 30%. This K(+)-enhanced recovery can be further potentiated by the binding of extracellular tetraethylammonium to the inactivated channel, although the tetraethylammonium does not interact directly with the K(+)-binding site. Our findings are consistent with a model in which K+ can bind and unbind slowly from a channel in the inactivated state, and inactivated channels that are bound by K+ will recover with a rate that is fast relative to unbound channels. Our data suggest that the kinetics of K+ binding to the modulatory site are slower than these recovery rates, especially at hyperpolarized voltages.

PMID:
8968586
PMCID:
PMC1233804
DOI:
10.1016/S0006-3495(96)79509-7
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center