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Electrophoresis. 1996 May;17(5):855-65.

Two-dimensional electrophoresis of plant proteins and standardization of gel patterns.

Author information

1
Research Institute for Biosciences, Science University of Tokyo, Noda, Japan.

Abstract

Proteins of two plants, Arabidopsis thaliana and rice (Oryza sativa) were subjected to two-dimensional electrophoresis analysis with two modifications: (i) comigration of external standard marker proteins with resultant horizontal and vertical stripes in the gel, and (ii) deblocking with a vapor of aqueous heptafluorobutyric acid for N-acetylserine. Approximately 5000 protein spots were separated from both the five tissues of Arabidopsis and the nine tissues of rice. Over one hundred spots were electroblotted for N-terminal sequencing. Among the newly sequenced proteins, 62 were from Arabidopsis and 51 from rice.

PMID:
8964270
DOI:
10.1002/elps.1150170507
[Indexed for MEDLINE]

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