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Biochem Pharmacol. 1997 Jan 10;53(1):89-93.

Inhibition of drug transport by genistein in multidrug-resistant cells expressing P-glycoprotein.

Author information

1
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston 77555-0641, USA.

Abstract

It has been claimed that the flavonoid genistein could be used to distinguish multidrug-resistant tumors expressing the multidrug resistance-associated protein (MRP) from those expressing P-glycoprotein (Pgp). Genistein would be block drug transport by MRP without affecting Pgp-mediated drug transport. However, we found that exposure to 200 microM genistein elicited an elevation in intracellular accumulation of rhodamine 123 (R123) and daunorubicin (DNR) in Pgp-expressing cell lines. Genistein inhibited R123 efflux in a rapidly reversible manner (ca. 2 min). The flavonoid also decreased photoaffinity labeling of Pgp by [3H]azidopine, a Pgp substrate. The present results show that genistein interacts with Pgp and inhibits Pgp-mediated drug transport. Hence, genistein cannot be used in simple assays to distinguish MRP- and Pgp-expressing cells.

PMID:
8960067
DOI:
10.1016/s0006-2952(96)00657-0
[Indexed for MEDLINE]

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