Format

Send to

Choose Destination
Br J Cancer. 1996 Dec;74(11):1828-33.

Characterisation of immune responses in pancreatic carcinoma patients after mutant p21 ras peptide vaccination.

Author information

1
Institute of Transplantation Immunology, National Hospital, University of Oslo, Norway.

Abstract

This is a study of immune responses generated by mutant ras peptide vaccination of patients with pancreatic adenocarcinoma. Responding T cells from one patient were cloned and two CD4+ T-lymphocyte clones (TLC) specific for the 12 Val peptide and restricted by HLA-DR6 or DQ2 were obtained. These class II molecules have not previously been found to bind or present mutant ras peptides to T cells. The DR6-restricted TLC showed marked cytotoxicity against autologous target cells pulsed with the 12 Val peptide. Target cells pulsed with the control peptide were not killed. Responding T cells from another patient showed cross-reactivity towards the homologous ras peptides. Investigation by limiting dilution analysis (LDA) revealed different T-cell precursor frequencies for the immunising, mutant ras peptide (1:28000), compared with the normal ras peptide (1:110000).

PMID:
8956801
PMCID:
PMC2077214
DOI:
10.1038/bjc.1996.638
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center