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Rapid Commun Mass Spectrom. 1996;10(3):335-40.

Identification and mapping of phosphocholine in animal tissue by static secondary ion mass spectrometry and tandem mass spectrometry.

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Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, TN 37831-6365, USA.


Secondary ion mass spectra and images were obtained from animal tissue samples using less than 10(13) primary ions/cm2. The mass spectra showed abundant peaks at m/z 184 and m/z 86. Tandem mass spectrometry (MS/MS) was used to identify the source of these ions as phosphocholine. Secondary ion images obtained using MS/MS were used to show that m/z 86 is an abundant gas-phase fragment ion derived from m/z 184. These results are discussed in terms of the physiology of the samples investigated.

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