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Cell. 1996 Nov 29;87(5):869-80.

Molecular mimicry in development: identification of ste11+ as a substrate and mei3+ as a pseudosubstrate inhibitor of ran1+ kinase.

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Department of Microbiology and Immunology, Morse Institute for Molecular Biology and Genetics, State University of New York Health Science Center at Brooklyn 11023, USA.

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  • Cell 1997 Jan 24;88(2):287.


ran1+ (pat1+) kinase inhibits exit from the mitotic cell cycle and entry into meiosis. Inactivation of ran1+ by mei3+ is sufficient to precipitate the entire meiotic developmental program. Here, we show that the ste11+ transcription factor is a substrate for ran1+ in vitro and that this reaction is directly inhibited by mei3+. Sequence comparison reveals that ste11+ contains two domains homologous to each other and to a domain of mei3+. Mutagenesis studies reveal that the regions of homology contain substrate specificity determinants. These results identify sequences critical for phosphorylation of ste11+ by ran1+ and suggest that mei3+ employs a pseudosubstrate mechanism for its inhibitory function.

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