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Plasmid. 1996 Jul;36(1):1-8.

Nucleic acid sequence and affiliation of pLUG10, a novel cadmium resistance plasmid from Staphylococcus lugdunensis.

Author information

1
Département de Recherche en Bactériologie Médicale (UPRES 1655), Faculté de Médecine R. Laennec, Lyon, France.

Abstract

Tolerance of Staphylococcus lugdunensis to relatively high levels of cadmium is mediated by a 3117-bp plasmid, pLUG10. Sequencing reveals three major open reading frames (ORFs) in a single orientation. One ORF encompasses the origin of replication and its predicted product (RepL: 350 amino acids (aa)) shows 70% homology in its deduced aa sequence with Rep proteins of the pT181 family. A lagging strand conversion signal (palA) very similar to that of class 1 plasmids is present outside the rep-ori locus. The other two ORFs of 209 and 116 aa show 92.5% homology between their deduced aa sequences and the CadB and CadX peptides from the pOX6 plasmid of Staphylococcus aureus. The CadX-like peptide is 40% homologous to the S. aureus CadC product. Deletion of the C-terminal cadX gene by restriction enzyme digestion or frame-shift inactivation of the cadB gene reduced, but did not completely abolish, cadmium resistance. The two gene products may act cooperatively to confer cadmium resistance in S. lugdunensis.

PMID:
8938046
DOI:
10.1006/plas.1996.0025
[Indexed for MEDLINE]

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