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FEMS Microbiol Lett. 1996 Nov 15;145(1):87-94.

Construction of GFP vectors for use in gram-negative bacteria other than Escherichia coli.

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1
Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA.

Abstract

A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn5gfp for making random promoter probe gfp insertions directly into host strains; p519gfp and p519nfp, broad host range mob+ plasmids containing gfp expressed from a lac and an npt2 promoter, respectively.

[Indexed for MEDLINE]

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