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Cell. 1996 Nov 15;87(4):721-32.

Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation.

Author information

1
Department of Biophysics, Boston University School of Medicine, Massachusetts 02218-2394, USA.

Abstract

The heterotrimeric Sec61p complex is a major component of the protein-conducting channel of the endoplasmic reticulum (ER) membrane, associating with either ribosomes or the Sec62/63 complex to perform co- and posttranslational transport, respectively. We show by electron microscopy that purified mammalian and yeast Sec61p complexes in detergent form cylindrical oligomers with a diameter of approximately 85 A and a central pore of approximately 20 A. Each oligomer contains 3-4 heterotrimers. Similar ring structures are seen in reconstituted proteoliposomes and native membranes. Oligomer formation by the reconstituted Sec61p complex is stimulated by its association with ribosomes or the Sec62/63p complex. We propose that these cylindrical oligomers represent protein-conducting channels of the ER, formed by ligands specific for co- and posttranslational transport.

PMID:
8929540
DOI:
10.1016/s0092-8674(00)81391-4
[Indexed for MEDLINE]
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