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Gene. 1996 Oct 31;178(1-2):125-30.

Construction by homologous recombination and phenotypic characterization of a DNA polymerase domain polA mutant of Mycobacterium smegmatis.

Author information

1
Molecular Biology Unit, South African Institute for Medical Research, Johannesburg, South Africa.

Abstract

Gene replacement was achieved by homologous recombination in Mycobacterium smegmatis (Ms) using a cloned segment of the polA gene (encoding the DNA polymerase I) disrupted within the region encoding the C-terminal DNA polymerase domain by a kanamycin-resistance marker. The Ms polA755:aph mutant thus generated displayed a phenotype of hypersensitivity to DNA damage induced by UV irradiation and by hydrogen peroxide challenge.

PMID:
8921903
DOI:
10.1016/0378-1119(96)00350-2
[Indexed for MEDLINE]

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