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Gene. 1996 Oct 24;177(1-2):275-6.

Nucleotide sequence of the Bacillus coagulans homologue of the spoIIA operon of Bacillus subtilis.

Author information

1
Department of Biochemistry, University of Oxford, UK.

Abstract

The spoIIA locus of Bacillus coagulans (Bc) was cloned into pTZ18R and the nucleotide sequence was determined. To clone the operon, one PCR primer corresponding to the C-terminal region of SpoIIAB, and a second corresponding to a region near the middle of SpoIIAC, were designed on the basis of the three previously published Bacillus spoIIA sequences. The Bc spoIIA sequence contains three open reading frames coding for putative proteins of 116, 147 and 252 aa.

PMID:
8921882
DOI:
10.1016/0378-1119(96)00306-x
[Indexed for MEDLINE]

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