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Ultramicroscopy. 1996 Jul;63(3-4):205-18.

A common-lines based method for determining orientations for N > 3 particle projections simultaneously.

Author information

1
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509, USA.

Abstract

A method is proposed for determining the directions of projections. An arbitrary number of projections of unknown three-dimensional structure are simultaneously used as input. The method is based on common lines and uses a new discrepancy measure accounting for the uneven distribution of common lines in angular space. An application to the 70S Escherichia coli ribosome data obtained from an energy-filtering electron microscope is described.

PMID:
8921628
[Indexed for MEDLINE]

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