A total of two hundred blood samples collected from patients presenting with febrile illness were followed by culture using traditional and biphasic culture methods. Use of biphasic medium was observed to be of dual advantage as it grew pathogens earlier (28.5 percent in 18 hours, 63 percent in 36 hours of incubation) as well as in more number of cultures i.e. higher isolation rate (78 percent) when compared to traditional method.