Format

Send to

Choose Destination
See comment in PubMed Commons below
Gene. 1996 Oct 17;176(1-2):93-6.

New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi.

Author information

1
Department of Plant Pathology, University of Wisconsin, Madison 53706, USA.

Abstract

New cosmid vectors were constructed for the ascomycete fungus, Magnaporthe grisea and the basidiomycete fungus, Ustilago maydis. These vectors are capable of transforming M. grisea at frequencies of up to 5 transformants/micrograms linear DNA and U. maydis at up to 25 transformants/microgram circular DNA for integrative transformation. In addition, 2800 transformants/microgram DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable.

PMID:
8918238
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center