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Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12840-4.

Repair of thalassemic human beta-globin mRNA in mammalian cells by antisense oligonucleotides.

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1
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599, USA.

Abstract

In one form of beta-thalassemia, a genetic blood disorder, a mutation in intron 2 of the beta-globin gene (IVS2-654) causes aberrant splicing of beta-globin pre-mRNA and, consequently, beta-globin deficiency. Treatment of mammalian cells stably expressing the IVS2-654 human beta-globin gene with antisense oligonucleotides targeted at the aberrant splice sites restored correct splicing in a dose-dependent fashion, generating correct human beta-globin mRNA and polypeptide. Both products persisted for up to 72 hr posttreatment. The oligonucleotides modified splicing by a true antisense mechanism without overt unspecific effects on cell growth and splicing of other pre-mRNAs. This novel approach in which antisense oligonucleotides are used to restore rather than to down-regulate the activity of the target gene is applicable to other splicing mutants and is of potential clinical interest.

PMID:
8917506
PMCID:
PMC24007
[Indexed for MEDLINE]
Free PMC Article
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