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Gene. 1996 Oct 10;175(1-2):281-3.

Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706.

Author information

1
Division of Infectious Diseases, University of Maryland School of Medicine, Baltimore 21201, USA.

Abstract

We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the alpha (catalytic) subunit of replicative DNA polymerase III (PolIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.

PMID:
8917113
DOI:
10.1016/0378-1119(96)00155-2
[Indexed for MEDLINE]

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