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Gene. 1996 Oct 10;175(1-2):105-8.

Isolation of a Candida glabrata centromere and its use in construction of plasmid vectors.

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Department of Mycology, Nippon Roche Research Center, Kanagawa, Japan.


A centromere has been isolated from Candida glabrata by functional selection based on the lethality of the SUP11 gene at high copy number. Nucleotide sequence analysis revealed a centromeric structure similar to that of Saccharomyces cerevisiae: the two highly conserved elements CDEI (8 bp) and CDEIII (26 bp) are separated by a 79-bp A+T-rich element, CDEII. Three centromere-bearing plasmid vectors with different selection markers have been constructed. These plasmids were highly stable in mitosis (< 1% loss rate per generation) and exist in one or two copies per cell.

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