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Mol Gen Genet. 1996 Oct 16;252(5):503-9.

Green fluorescent protein (GFP) as a new vital marker in the phytopathogenic fungus Ustilago maydis.

Author information

1
Institut für Genetik und Mikrobiologie, Universität München, Germany.

Abstract

Pathogenic development of Ustilago maydis, the causative agent of corn smut disease, is a multistep process. Compatible yeast-like cells fuse and this generates the infectious dikaryon which grows filamentously. Having entered the plant the dikaryon induces tumors in its host in which massive proliferation of fungal material, karyogamy and spore formation occur. In order to follow fungal development from the initial steps to the final stage we have expressed the green fluorescent protein (GFP) from Aequorea victoria as a vital marker in U. maydis and demonstrate that GFP-tagged strains can be used to study host-pathogen interactions in vivo.

PMID:
8914511
DOI:
10.1007/bf02172396
[Indexed for MEDLINE]

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