Humoral immune response against the mycobacterial antigen A60 was evaluated in 38 subjects: 13 healthy volunteers (Group I), 10 patients with a defined acute or chronic non tuberculous lung disease (Group II), 15 patients suffering from pulmonary tuberculosis (Group III). Saliva IgA in samples diluted in various concentrations (1:10, 1:30, 1:50) and serum IgG and IgA levels were measured by ELISA. Positive values of IgG were found in sera of 0/13 subjects from Group I, 1/10 from Group II, 12/15 from Group III; searching for IgA in serum was positive in 1/13 subjects from Group I, 2/10 from Group II, 11/15 from Group III, 1:30 dilution of saliva led to positive results in 0/13 subjects from Group I, 0/10 from Group II and 10/15 from Group III. The measurement of anti-A60 IgA levels in both saliva and serum might be a useful complement to serology based on detection of anti-A60 IgG in blood samples.