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Antimicrob Agents Chemother. 1996 Nov;40(11):2562-6.

Detection of erythromycin-resistant determinants by PCR.

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1
Department of Infectious Diseases, Pfizer, Inc., Groton, Connecticut 06340, USA. Joyce_A_Sutcliffe@groton.pfizer.com

Abstract

Erythromycin resistance determinants include Erm methylases, efflux pumps, and inactivating enzymes. To distinguish the different mechanisms of resistance in clinical isolates, PCR primers were designed so that amplification of the partial gene products could be detected in multiplex PCRs. This methodology enables the direct sequencing of amplified PCR products that can be used to compare resistance determinants in clinical strains. Further, this methodology could be useful in surveillance studies of erythromycin-resistant determinants.

PMID:
8913465
PMCID:
PMC163576
[Indexed for MEDLINE]
Free PMC Article
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