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Am J Ophthalmol. 1996 Nov;122(5):732-5.

Polymerase chain reaction detection of herpes simplex virus in tear fluid from atypical herpetic epithelial keratitis after penetrating keratoplasty.

Author information

1
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.

Abstract

PURPOSE:

We investigated the efficacy of polymerase chain reaction (PCR) in diagnosing atypical epithelial keratitis after a penetrating keratoplasty (PKP) for herpetic corneal scarring.

METHODS:

We examined three eyes of three patients with keratitis after a PKP for herpetic corneal scarring. The clinical presentation of the keratitis was atypical. DNA extracted from the tears was subjected to PCR and Southern blot hybridization.

RESULTS:

Herpes simplex virus DNA was identified in all cases of presumptive recurrent herpetic keratitis.

CONCLUSION:

Polymerase chain reaction is rapid and effective for diagnosing atypical recurrent herpetic keratitis.

PMID:
8909218
[Indexed for MEDLINE]

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