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Genome Res. 1996 Oct;6(10):986-94.

Real time quantitative PCR.

Author information

1
BioAnalytical Technology Department, Genentech, Inc., South San Francisco, California 94080, USA.

Abstract

We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.

PMID:
8908518
[Indexed for MEDLINE]
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