Format

Send to

Choose Destination
See comment in PubMed Commons below
J Chromatogr B Biomed Appl. 1996 Sep 20;684(1-2):307-21.

Separation and functional analysis of eukaryotic DNA topoisomerases by chromatography and electrophoresis.

Author information

1
Department of Molecular and Structural Biology, Univ. Aarhus, Denmark.

Abstract

DNA topoisomerases are enzymes that control DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through the transient gaps. Consequently, these enzymes play a crucial role in the regulation of the physiological function of the genome. Beyond their normal functions, topoisomerases are important cellular targets in the treatment of human cancers. In this review we summarize current protocols for extracting and purifying DNA topoisomerases, and for separating subtypes and isoforms of these enzymes. Furthermore, we discuss methods for measuring the catalytic activity of topoisomerases and for monitoring the molecular effects of topoisomerase-directed antitumor drugs in cell-free assays.

PMID:
8906479
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center