Remnant-like particles (RLP) from NIDDM patients with apolipoprotein E3/3 phenotype stimulate cholesteryl ester synthesis in human monocyte-derived macrophages

Artery. 1996;22(3):155-63.

Abstract

We tested the ability of remnant-like particles (RLP) from NIDDM patients to stimulate cholesteryl ester synthesis in human monocyte-derived macrophages. Six NIDDM patients were studied together with 7 non-diabetic subjects. All had apolipoprotein (apo) E3/3 phenotype. RLP were isolated using an immunoaffinity gel mixture of anti apo B-100 and anti apo A-1 monoclonal antibodies coupled to Sepharose 4B. Plasma levels of triglyceride, total cholesterol (chol) and high density lipoprotein-chol were not statistically different, but plasma levels of RLP-chol were significantly (p < 0.05) higher in NIDDM patients (10.5 +/- 2.2 mg/dl) than in non-diabetic controls (5.0 +/- 1.7 mg/dl). The effects of RLP from NIDDM patients on macrophage cholesteryl ester synthesis were estimated. 14C-oleate incorporation into cholesteryl esters in macrophages was significantly (p < 0.01) higher in NIDDM patients with apo E3/3 (0.326 +/- 0.037 nmole/mg cell protein) than in non-diabetic controls with apo E3/3 (0.181 +/- 0.011 nmole/ mg cell protein). It is suggested that RLP from NIDDM play a role in the accumulation of cholesteryl esters and are one of the risk factors for the acceleration of atherosclerosis in NIDDM.

MeSH terms

  • Antibodies, Monoclonal
  • Apolipoprotein A-I / immunology
  • Apolipoprotein B-100
  • Apolipoprotein E3
  • Apolipoproteins B / immunology
  • Apolipoproteins E / blood
  • Apolipoproteins E / genetics*
  • Apolipoproteins E / isolation & purification
  • Cells, Cultured
  • Cholesterol / blood
  • Cholesterol Esters / biosynthesis*
  • Cholesterol, HDL / blood
  • Chromatography, Affinity
  • Diabetes Mellitus, Type 2 / blood*
  • Diabetes Mellitus, Type 2 / genetics*
  • Female
  • Genotype
  • Humans
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Male
  • Monocytes
  • Oleic Acid / metabolism
  • Phenotype
  • Reference Values
  • Triglycerides / blood

Substances

  • Antibodies, Monoclonal
  • Apolipoprotein A-I
  • Apolipoprotein B-100
  • Apolipoprotein E3
  • Apolipoproteins B
  • Apolipoproteins E
  • Cholesterol Esters
  • Cholesterol, HDL
  • Triglycerides
  • Oleic Acid
  • Cholesterol