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Gene. 1996 Oct 3;174(2):315-8.

Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe.

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Howard Hughes Medical Institute, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA.


The construction of vectors for use in Schizosaccharomyces pombe using the his3+ gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.

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