A minisatellite polymorphism in intron III of the barramundi (Lates calcarifer) growth hormone gene

Genome. 1996 Oct;39(5):934-40. doi: 10.1139/g96-117.

Abstract

This paper describes the detection of a polymorphism within the growth hormone (GH) gene of the fish barramundi (Lates calcarifer). PCR amplification of barramundi genomic DNA generated three different sized products: A, 409 bp; B, 478 bp; and H, 520 bp. Each barramundi isolate displayed one of four different types of profiles, which contained specific combinations of these PCR products. Sequence analysis confirmed that products A and B are different forms of the barramundi GH gene, and studies showed that product H was an artifact due to heteroduplex formation between the two smaller-sized molecules. The polymorphic nature of these PCR products was due to differences in the number of repeat monomers within the 5' end of the barramundi decaminisatellite, an AT-rich repetitive sequence that was identified within intron III of this gene. The barramundi decaminisatellite consisted of 24 or 28 10-nucleotide imperfect direct repeat monomers in a tandem array. The monomers were grouped into one of three different families and evidence for monomer homogenization by crossover fixation was presented. The barramundi decaminisatellite differed from previously reported AT- or GC-rich minisatellites, although a similar decaminisatellite has been identified in intron III of the tilapia GH gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Fishes / genetics*
  • Genes
  • Growth Hormone / genetics*
  • Introns
  • Minisatellite Repeats
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Alignment

Substances

  • Growth Hormone

Associated data

  • GENBANK/M84774
  • GENBANK/U16816