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EMBO J. 1996 Sep 16;15(18):5085-92.

The initiator protein E1 binds to the bovine papillomavirus origin of replication as a trimeric ring-like structure.

Author information

1
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Abstract

The replication initiator protein E1 binds to the origin of replication of bovine papillomavirus in several forms. E1 can bind to its recognition sequence as a monomer together with the viral transcription factor E2, or as a trimeric E1 complex. The trimerization of E1 is mediated by the sequence-specific binding of E1 to DNA, and results in an E1 complex that is linked topologically to the DNA because the three molecules of E1 form a ring-like structure that encircles the DNA. These results demonstrate that E1 utilizes unusual mechanisms for sequence-specific binding to DNA and for the generation of a structure that encircles the DNA. We believe that these forms of E1 bound to the origin of replication represent intermediates in a transition in the function of E1, from a sequence-specific origin of replication recognition protein to a form of E1 that is competent for the initiation of viral DNA replication.

PMID:
8890182
PMCID:
PMC452248
[Indexed for MEDLINE]
Free PMC Article

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