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Mol Biotechnol. 1996 Aug;6(1):87-9.

Simple procedure for estimating the efficiency of PCR.

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1
Metatron Inc., New York, NY 10003, USA.

Abstract

The extension efficiency of PCR-driven DNA amplification was examined by step-controlled limiting dilution method using as internal controls 8E5 lymphocytes carrying a single copy of HIV genome. The results reveal that under standard conditions recommended by the PCR kit manufacturer the exponential growth of DNA template is approximately one third of the theoretical doubling rate.

PMID:
8887366
DOI:
10.1007/BF02762328
[Indexed for MEDLINE]

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