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Clin Lab Med. 1996 Mar;16(1):89-103.

NASBA: a novel, isothermal detection technology for qualitative and quantitative HIV-1 RNA measurements.

Author information

1
Department of Cell Biology, Advanced BioScience Laboratories, Inc., Kensington, Maryland, 20855, USA.

Abstract

Although immunoassays have long served as the standard in the field of diagnostics, the advent of nucleic acid amplification technologies allows for a new array of diagnostic applications. NASBA, nucleic acid sequence-based amplification, is one such technology that is highly suited for the amplification of RNA. As such, NASBA is applied readily as a diagnostic tool for infectious diseases, particularly for RNA viruses, such as retroviruses. The development and application of NASBA technology as a qualitative and quantitative diagnostic system for HIV-1 are described in this article.

PMID:
8867585
[Indexed for MEDLINE]

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