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Biochem Biophys Res Commun. 1996 Oct 3;227(1):20-6.

Inhibition of Raf/MAPK signaling in Xenopus oocyte extracts by Raf-1-specific peptides.

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1
Institut für Medizinische Virologìe, Universität Zürich, Gloriastrasse, Switzerland.

Abstract

Raf-1 is an upstream element of the mitogen-activated protein kinase (MAPK) pathway which leads to cell proliferation and differentiation. In this study Raf-1 derived peptides comprising the conserved amino acid residues Arg89 and Ser259, involved in binding of activated Ras and 14-3-3 proteins, respectively, were shown to interfere with MAPK activation in extracts from immature Xenopus oocytes. Lipids prepared from oocyte extracts can stimulate MAPK in a Ras- and protein kinase C-independent manner. This lipid-induced MAPK activation is blocked by a Raf-1 derived peptide comprising Ser259.

PMID:
8858097
DOI:
10.1006/bbrc.1996.1461
[Indexed for MEDLINE]

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