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Prenat Diagn. 1996 Jul;16(7):615-21.

Rapid in situ harvesting and cytogenetic analysis of perinatal tissue samples.

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Wessex Regional Genetics Laboratory, Salisbury District Hospital, Wiltshire, U.K.


Thirty perinatal solid tissue samples were used in a pilot study to test the efficacy of collagenase disaggregation onto coverslips, open system culture under low O2 conditions, pre-harvest incubation in bromodeoxyuridine (BrdU) and colcemid, and in situ automated harvesting. Following the success of the pilot study, the new method was applied to a further 126 consecutive diagnostic samples making a total of 156. The method reduced average tissue culture times from 17 to 8.7 days (range 2-17), improved success rates from 76 to 88 per cent, and simultaneously increased the resolution of cytogenetic analysis.

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