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Virus Res. 1995 Dec;39(2-3):221-35.

Characterisation of the interaction between the nucleoprotein and phosphoprotein of pneumonia virus of mice.

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Department of Biological Sciences, University of Warwick, Coventry, UK.


A protein blotting technique was used to study the interaction occurring between the pneumonia virus of mice N protein and other PVM encoded proteins expressed in infected cells. Measurement of the degree of binding indicated that the N protein specifically interacted only with the full-length 39 kDa P protein in infected cells. Truncated N-related proteins were synthesised in vitro and incubated with filter-bound full-length and truncated P proteins. The data suggested that many regions of the N protein are cooperatively involved in the binding process. It was also determined that both the amino and the carboxyl-terminal regions of the PVM P protein were essential for binding to N protein.

[Indexed for MEDLINE]

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