Format

Send to

Choose Destination
Nucleic Acids Res. 1996 Sep 15;24(18):3649-50.

A PCR-based DNA fingerprinting technique: AFLP for molecular typing of bacteria.

Author information

1
Agricultural Biotechnology R&D, Life technologies, Inc., GIBCO BRL, Gaithersburg, MD 20877, USA.

Abstract

Amplified restriction fragment polymorphism (AFLP) is a PCR-based DNA fingerprinting technique. In AFLP analysis, bacterial genomic DNA is digested with restriction enzymes, ligated to adapters, and a subset of DNA fragments are amplified using primers containing 16 adapter defined sequences with one additional arbitrary nucleotide. Polymorphisms of different Escherichia coli strains or Agrobacterium tumefaciens strains were demonstrated as distinct, unique bands in a denaturing sequencing gel using AFLP. The polymorphisms detected between BL21 and BL21F'IQ and between DH5 alpha and DH5 alpha F'IQ strains indicated that AFLP is able to resolve differences in F' episomal DNA (approximately 100 kb).

PMID:
8836198
PMCID:
PMC146140
DOI:
10.1093/nar/24.18.3649
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center