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Poult Sci. 1996 Feb;75(2):254-6.

Cryopreservation of rooster semen in thirteen and sixteen percent glycerol.

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BioPore, Inc., State College, Pennsylvania 16805, USA.


Semen from Barred Plymouth Rock roosters was cryopreserved with glycerol concentrations of 13 and 16% in a microprocessor-controlled freezer. Thawing and deglycerolation were facilitated by the use of an improved microprocessor-controlled thawing device and high speed dialyzer. Deglycerolated semen (100 mu L; 192 and 154 million sperm, respectively, for the 13 and 16% glycerol concentration) was inseminated into Single Comb white Leghorn hens. Three inseminations were done at 4-d intervals. Eggs were collected for 10 d starting 1 d after the first insemination, and incubated for 4th d. Fertility was determined by candling after the 4th d. Fertility measurements of 62.4 and 65% were obtained from the sperm frozen in 13 and 16% glycerol concentrations, respectively, for the 10-d period.

[Indexed for MEDLINE]

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