Format

Send to

Choose Destination
Poult Sci. 1996 Feb;75(2):254-6.

Cryopreservation of rooster semen in thirteen and sixteen percent glycerol.

Author information

1
BioPore, Inc., State College, Pennsylvania 16805, USA.

Abstract

Semen from Barred Plymouth Rock roosters was cryopreserved with glycerol concentrations of 13 and 16% in a microprocessor-controlled freezer. Thawing and deglycerolation were facilitated by the use of an improved microprocessor-controlled thawing device and high speed dialyzer. Deglycerolated semen (100 mu L; 192 and 154 million sperm, respectively, for the 13 and 16% glycerol concentration) was inseminated into Single Comb white Leghorn hens. Three inseminations were done at 4-d intervals. Eggs were collected for 10 d starting 1 d after the first insemination, and incubated for 4th d. Fertility was determined by candling after the 4th d. Fertility measurements of 62.4 and 65% were obtained from the sperm frozen in 13 and 16% glycerol concentrations, respectively, for the 10-d period.

PMID:
8833379
DOI:
10.3382/ps.0750254
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center