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Anal Biochem. 1996 Mar 15;235(2):113-8.

The use of diethylaminoethyl-cellulose-membrane filters in a bioassay to quantify melanin synthesis.

Author information

1
L'Oreal, Centre de Recherche Charles Zviak, France.

Abstract

Melanins are a class of extremely insoluble granular biopolymers with a yet ill-defined chemical structure, properties which render the isolation and quantification of these molecules very difficult. Based on their strong anionic character, however, a biophysical property shared by all melanins, we have developed a method allowing total recovery of the pigment. Soluble melanin is bound and granular melanin retained by DEAE-cellulose membrane filters. This approach provides an excellent means to quantify melanin synthesis in normal human melanocytes alone and in coculture with normal human keratinocytes.

PMID:
8833318
DOI:
10.1006/abio.1996.0102
[Indexed for MEDLINE]

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