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Adv Exp Med Biol. 1995;380:529-35.

Characterization of the transmissible gastroenteritis virus (TGEV) transcription initiation sequence. Characterization of TGEV TIS.

Author information

1
Division of Molecular Biology, Institute for Animal Health, Newbury, Berkshire, United Kingdom.

Abstract

The ability of the TGEV transcription initiation sequence (TIS) to produce subgenomic RNAs was investigated by placing a reporter gene, chloramphenicol acetyltransferase (CAT) under the control of either the mRNA 6 or the mRNA 7 TISs. Both constructs only produced CAT in TGEV infected cells and the amount of CAT produced from the mRNA 7 TIS was less than from the mRNA 6 TIS. Mutations were made within and around the TISs and the effect on CAT production assayed. THe results showed that the TGEV TIS acted as a initiator of transcription for CAT, though the degree of base pairing between the TIS and leader RNA was not the only factor implicated in the control transcription.

PMID:
8830536
DOI:
10.1007/978-1-4615-1899-0_84
[Indexed for MEDLINE]

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