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Microsc Res Tech. 1996 Jan 1;33(1):73-86.

Applications of laser scanning microscopy for analysis of aquatic microhabitats.

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Section of Microbiology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853, USA.


Laser scanning microscopy was used 1) to analyze Mn oxide-encrusted biofilms and particles in marine Mn-oxidizing enrichment cultures, 2) to optimize fluorescence in situ hybridization protocols of 16S ribosomal RNA-targeted oligonucleotide signature probes for single bacterial cell identification in particles from a wetland, and 3) to develop a combined immunofluorescence-microautoradiography procedure for analysis of the distribution of 14C-labeled organic compounds and 14C-mineralizing bacteria in groundwater seep sediments. The results demonstrated the wide applicability and benefits of using laser scanning microscopy for analysis of complex microbial assemblages.

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