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Protein Expr Purif. 1996 Sep;8(2):254-61.

Overexpression in Pichia pastoris and crystallization of an elicitor protein secreted by the phytopathogenic fungus, Phytophthora cryptogea.

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1
Unité de Recherche de Biochimie et Structure des Protéines, INRA, Domaine de Vilvert, Jouy-en-Josas Cedex, F-78352, France.

Abstract

A synthetic gene encoding beta-cryptogein, a member of the elicitin family, has been cloned into a vector for expression by the methylotrophic yeast, Pichia pastoris. Having first optimized the gene construction for secretion, we have overexpressed a modified beta-cryptogein in a secreted form. A purification scheme suited to this expression system has been developed and highly pure, biologically active protein has been obtained. For structural analysis of this recombinant beta-cryptogein, and new mutated forms thereof, optimal conditions for the crystallization of this protein have been determined and crystals that diffract to 2.2 A have been obtained.

PMID:
8812874
DOI:
10.1006/prep.1996.0098
[Indexed for MEDLINE]
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