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Plasmid. 1996 May;35(3):164-73.

Functional comparison of conjugative transposons Tn916 and Tn925.

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Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames 50011, USA.


During interspecies matings between Bacillus subtilis and Bacillus thuringiensis subsp. israelensis, transfer of conjugative transposon Tn916 was detected at a frequency of 1.1 x 10(-4) transconjugants per donor. Tn916-dependent transfer of plasmids pC194 and pE194 was detected at frequencies of 1.4 x 10(-5) and 3.2 x 10(-7) transconjugants per donor, respectively. Similar frequencies were obtained during parallel matings with otherwise isogenic strains that contain Tn925 instead of Tn916. Tn916- or Tn925-dependent transfer of plasmids pC194 or pUB110 from the recipient to the donor (retrotransfer) was not observed during inter- or intraspecies matings. Transposon-mediated plasmid transfer by Tn916 and Tn925 is a Rec independent event. Thus, the data from studies in which otherwise isogenic donor and recipient strains were used indicated that Tn916 and Tn925 are, from a functional point of view, much more similar than previously suggested.

[Indexed for MEDLINE]

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