Format

Send to

Choose Destination
Nucleic Acids Res. 1996 Sep 1;24(17):3469-71.

One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins.

Author information

1
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.

Abstract

With the availability of the complete yeast genomic sequence, techniques which allow the rapid functional analysis of genes of interest are of increasing importance. Here we report a technique which allows the initial characterisation of genes of interest, through the construction of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR.

PMID:
8811105
PMCID:
PMC146098
DOI:
10.1093/nar/24.17.3469
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center