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FEMS Microbiol Lett. 1996 Sep 1;142(2-3):231-5.

In vivo and in vitro analysis of Bordetella pertussis catalase and Fe-superoxide dismutase mutants.

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1
Laboratoire des Bordetella, Institut Pasteur, Paris, France.

Abstract

Bordetella pertussis produces a catalase and a Fe-superoxide dismutase. The importance of these enzymes in virulence was investigated, in vitro as well as in vivo, by using mutants deficient in their production. The catalase-deficient mutant survived within polymorphonuclear leukocytes, killed J774A.1 macrophages through apoptosis, and behaved as the parental strain in a murine respiratory infection model. These results suggest no direct role for catalase in B. pertussis virulence. The absence of expression of Fe-superoxide dismutase had profound effects on the bacterium including a reduced ability to express adenylate cyclase-hemolysin and pertactin, two factors important for B. pertussis pathogenesis. The Fe-superoxide dismutase-deficient mutant also had decreased abilities to colonize and persist in the murine respiratory infection model.

[Indexed for MEDLINE]

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