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FEMS Immunol Med Microbiol. 1996 Jun;14(2-3):167-77.

Factors affecting the immunogenicity of tetanus toxin fragment C expressed in Lactococcus lactis.

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1
Department of Pathology, University of Cambridge, UK.

Abstract

The relative immunogenicity of tetanus toxin fragment C (TTFC) has been determined in three different strains of inbred mice when expressed in Lactococcus lactis as a membrane-anchored protein (strain UCP1054), as an intracellular protein (strain UCP1050), or as a secreted protein which is partly retained within the cell wall (strain UCP1052). Protection against toxin challenge (20 x LD50) could be obtained without the induction of anti-lactococcal antibodies. When compared in terms of the dose of expressed tetanus toxin fragment C required to elicit protection against lethal challenge the membrane-anchored form was significantly (10-20 fold) more immunogenic than the alternative forms of the protein.

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