Format

Send to

Choose Destination
Chem Biol. 1996 Jun;3(6):471-7.

Artificial antibodies to corticosteroids prepared by molecular imprinting.

Author information

1
Pure and Applied Biochemistry, Chemical Center, Lund University, P.O. Box 124, S-22100 Lund, Sweden. Olof.Ramstrom@tbiokem.lth.se

Abstract

BACKGROUND:

Molecular imprinting can be used to prepare antibody and receptor mimics. We have previously shown that acrylic acid polymers can be imprinted to recognize a variety of small molecules. Here, we show that molecularly imprinted polymers (MIPs) can selectively recognize steroid structures.

RESULTS:

Artificial antibodies mimicking the binding performance of natural anti-corticosteroid antibodies have been prepared using a molecular imprinting protocol with either cortisol or corticosterone as a target molecule. The binding characteristics of a range of structurally related ligands were estimated using a form of radioimmunoassay. The antibody mimics were found to be highly selective for the ligands used in their preparation and the cross-reactivities with compounds of related structure resembled those obtained in studies with natural antibodies.

CONCLUSIONS:

The binding properties of MIPs, prepared against corticosteroids, exhibit strong similarities to those of naturally raised antibodies. Such artificial antibodies may serve as a useful complement to their natural counterparts in studies of corticosteroid binding events.

PMID:
8807877
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center