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Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9975-9.

Stable transfer of intact high molecular weight DNA into plant chromosomes.

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  • 1Plant Science Center, Cornell University, Ithaca, NY 14853, USA.

Abstract

In conjunction with an enhanced system for Agrobacterium-mediated plant transformation, a new binary bacterial artificial chromosome (BIBAC) vector has been developed that is capable of transferring at least 150 kb of foreign DNA into a plant nuclear genome. The transferred DNA appears to be intact in the majority of transformed tobacco plants analyzed and is faithfully inherited in the progeny. The ability to introduce high molecular weight DNA into plant chromosomes should accelerate gene identification and genetic engineering of plants and may lead to new approaches in studies of genome organization.

PMID:
8790442
PMCID:
PMC38540
[PubMed - indexed for MEDLINE]
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