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Glycoconj J. 1996 Feb;13(1):53-6.

Large scale preparation of PA-oligosaccharides from glycoproteins using an improved extraction method.

Author information

1
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd. Fukuura, Japan.

Abstract

We have developed a new method for the large scale preparation of pyridylaminated (PA-) oligosaccharides from glycoproteins. Phenol/chloroform extraction was adapted for the removal of protein and excess 2-aminopyridine, improving the efficiency of preparation. From a 2.5 g sample of human apo-transferrin, 25-30 mu mol of agalacto biantennary PA-oligosaccharide could be obtained. By increasing the concentration of PA-oligosaccharide substrate, we were able to detect a very low level of N-acetylglucosaminlytransferase IV activity in CHO cell extracts.

PMID:
8785488
[Indexed for MEDLINE]

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