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Nat Struct Biol. 1996 Sep;3(9):796-802.

ADP release produces a rotation of the neck region of smooth myosin but not skeletal myosin.

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Graduate Group in Biophysics, University of California, San Francisco 94143-0448, USA.


Current theories of muscle cross-bridge function suggest that force is generated by a change in the orientation of the myosin neck region. We attached a paramagnetic probe to a subunit in the neck region and measured the orientation of the probe using electron paramagnetic resonance spectroscopy. The angle of the probes on smooth myosin S1 were changed by 20 degrees +/- 4 degrees on addition of ADP (50% effect at 5 +/- 2 microM), but ADP produced little effect on skeletal S1. The orientation of smooth myosin, +ADP, resembled that of skeletal myosin, +/- ADP, suggesting that the release of ADP generates an extra rotation of the neck region in smooth muscle at the end of its power stroke.

[Indexed for MEDLINE]

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