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Genetics. 1996 Jan;142(1):173-7.

Germline transformation of Drosophila virilis mediated by the transposable element hobo.

Author information

1
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

Abstract

A laboratory strain of Drosophila virilis was genetically transformed with a hobo vector carrying the miniwhite cassette using a helper plasmid with an hsp70-driven hobo transposase-coding sequence. The rate of transformation was 0.5% per fertile GO animal. Three transgenic insertions were cloned and characterized and found to be authentic hobo insertions. These results, together with the known widespread distribution of hobo in diverse insect species, suggest that hobo and related transposable elements may be of considerable utility in the germline transformation of insects other than D. melanogaster.

PMID:
8770594
PMCID:
PMC1206945
[Indexed for MEDLINE]
Free PMC Article

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